Recombinant bovine basic fibroblast growth factor gel formulation(Zhuhai Essex Bio-Pharmaceutical Co., Ltd.)

Tympanic membrane perforation (TMP) caused by blast injury result in poor TMP closure and pathological regeneration of the tympanic membrane (TM). TM regeneration therapy using basic fibroblast growth factor (bFGF) has demonstrated good outcomes for chronic otitis media.

To investigate the efficacy of bFGF treatment in the management of blast-induced TMP.

Male CBA/J mice (10-12-week-old) were divided into the bFGF-treatment and no-treatment groups. Blast-tube systems were used to induce TMP. Auditory evaluation, TM observation, histological evaluation of the regenerated TM, and histological evaluation of the inner ear were conducted.

Auditory assessment revealed no difference between the groups, with persistent auditory threshold elevation being observed even 1 month post-injury. Similarly, histological evaluation of the inner ear hair cells revealed no significant differences between the groups; no hair cell death was evident. However, the regenerated TM in the bFGF-treated group was significantly thicker than that in the untreated group.

A good TMP closure rate was observed in the present study; however, bFGF treatment exacerbated thickening of the regenerated TM. Future studies must study the therapeutic effects of bFGF treatment using various animal models and timing and concentration of bFGF treatment.

Background: Diabetic wound is one of the most common diabetic chronic complications. Effective treatments of diabetic wound remain limited. Here, we explored the effects of basic fibroblast growth factor (bFGF)-acellular dermal matrix (ADM) hydrogel on the diabetic wound. Methods: The bFGF-ADM hydrogel was manufactured by mixing 180 µL ADM hydrogel and 20 µL bFGF aqueous solution (10 mg/mL). The morphology of ADM hydrogel and bFGF-ADM hydrogel was observed under scanning electron microscope. The release property of bFGF-ADM hydrogel was determined by ELISA. CCK-8 assay was utilized to estimate the cell viability of mouse skin fibroblasts. The diabetes mellitus (DM) model was established in rats. The four wounds on the back of each DM rat were treated with the ADM hydrogel, bFGF-ADM hydrogel, bFGF aqueous solution and no solution (control), respectively. The wound healing rate of each rat was estimated. The traumatized skin tissue of each rat was observed by H&E staining and Sirius red staining. Results: The bFGF-ADM hydrogel displayed an interconnected pore structure and bFGF was gradually released from the bFGF-ADM hydrogel over time. The bFGF-ADM hydrogel could enhance the cell viability of skin fibroblasts and promote the wound healing rate, the re-epithelialization of wound and increase the collagen fiber content of dermis. And the bFGF-ADM hydrogel exhibited better therapeutic effects of diabetic wound than either bFGF or ADM alone. Conclusions: Our study revealed that the bFGF-ADM hydrogel could promote diabetic wound healing.