CM11

Antimicrobial peptides (AMPs) have received attention in recent years for dealing with wound infections. This study aimed to evaluate the antibacterial activity of antimicrobial peptides FR7 and CM11 against methicillin-resistant Staphylococcus aureus (MRSA) strains in vitro and investigate their wound healing properties in vivo.

The minimum inhibitory concentrations (MIC) of these peptides were determined using the microdilution method. Both peptides were evaluated for toxicity in L929 cells. Their antimicrobial activity and wound healing potency were tested in vivo. Wounds infected with MRSA were treated with the peptide gel or Mupirocin (positive control) or untreated (negative control). Wound healing was assessed by measuring wound contraction (days 3, 7, 10, 14, 17 and 21) and histopathological changes (days 3, 7, 14 and 21).

MRSA strain was inhibited by both AMPs at 8 μg/ml with minimal cytotoxicity to L929 cells. In vivo analysis showed higher wound contraction in the AMPs-treated groups compared to controls, with CM11 showing better results than FR7. Histopathological analysis indicated better wound healing in the CM11-treated group.

This study demonstrates the antibacterial and wound healing potential of CM11 and FR7 peptides, with CM11 showing greater efficacy and potential for developing treatments for wound infections, especially those caused by antibiotic-resistant strains.

This study aimed to investigate the effects of zoledronic acid (ZA) and antibacterial CM11 peptide on the osteoinduction and antibacterial properties of bioactive glass (BG). The bioactive glass/gelatin (BG/Gel) composite was synthesized using the sol-gel method. The 2-x minimum inhibitory concentration of the peptide and 4.00 mg mL^-1 of ZA were added to the BG/Gel during fabrication. The BG/Gel composite morphological and structural characteristics and anti-bacterial activities were analyzed using Fourier transform infra-red spectroscopy, scanning electron microscopy and disk diffusion test, respectively. The release of the peptide and ZA from BG/Gel was measured using ultra-violet spectroscopy. After 14 days, the effects of the peptide/ ZA-containing BG/Gel (PZ-BG/Gel) on the growth and differentiation of mesenchymal stem cells were evaluated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, calcium and alkaline phosphatase assays, immunocytochemical staining for osteocalcin (OCN) and real-time polymerase chain reaction for OCN, type I collagen, bone morphogenetic protein 2 and Runt-related transcription factor-2 genes. The disk diffusion test showed the anti-microbial activity of the scaffold against multi-drug-resistant isolates of Pseudomonas aeruginosa and Staphylococcus aureus. Analyses showed a significantly higher level of stem cells differentiation into the osteo-genic cells in PZ-BG/Gel scaffold compared to BG/Gel scaffold alone. Accordingly, osteoblast markers were significantly increased in comparison with the control. In conclusion, the osteo-induction and antibacterial properties of BG-based scaffold can be improved using ZA and CM11.