Seven previously undescribed metabolites, including four cladosporols, named cytosporols A-C (1-3), cladosporol L (4), and three polyketides, photinide G (5), cytorhizophin K (7), pestalotiotone C (9), were isolated from the cultures of the mangrove-derived fungus Cytospora heveae NSHSJ-2. The planar structures of the undescribed compounds were determined by analyzing their detailed spectroscopic data. The absolute configurations were further determined through electronic circular dichroism calculations, the modified Mosher's method, NMR calculations and DP4+ analysis. In the bioassay, cytosporol C (3) displayed cytotoxic activities against MDA-MB-435, MDA-MB-231, A549, HepG2 and HCT-116 cell lines with IC50 values ranging from 7.7 to 31.4 μM. Meanwhile, cytosporol C (3) revealed DPPH· scavenging activity with the EC50 value of 47.6 μM.